摘要
目的分析25株矽肺结核分枝杆菌耐卡那霉素和Km基因突变情况。方法通过传统梯度药敏试验,聚合酶链反应-单链构象多态性(PCR-SSCP)和测序,分析临床耐药株Km基因的变化。结果矽肺结核药敏试验,卡那霉素耐药率为28%(7/25),25株临床分离株的16SrDNASSCP电泳图谱均与结核分枝杆菌标准相同,7株耐卡那霉素分离株中,2株29.6%(2/7)Km基因SSCP泳动异常。结论抗结核二线药中,卡那霉素已出现耐药,其部分耐药原因可能是由于分枝杆菌耐卡那霉素的Km基因突变所致。
Objective To analyze kanamycin drug resistance and its Km gene mutation in 25 mycobacterium trbereulosis strains isolated from pulmonary silicotuberculosis. Methods All isolates were detected by traditional drug susceptibility test and all Km genes were detected by plymerase chain reaction-single strand conformation polymorphism（PCR-SSCP） and sequencing. Results We fonud that 7 isolates were resistant to kanamycin in 25 isolates. They had the same SSCP electrophpresis maps of 16S rDNA conpared with standard strain of mycobacterium tuberculosis. Moreover,2 isolates resistance to kanamycin showed different SSCP electrophoresis maps in 7 kanamycin resistant isolates. Conclusion The strains resistance to kanamycin have emerged. The Km gene mutation maybe contributes partially to kanamycin resistance in mycobacteria.
出处
《临床肺科杂志》
2010年第1期24-25,共2页
Journal of Clinical Pulmonary Medicine
关键词
矽肺结核
卡那霉素
Km基因
耐药
silicotuberculosis
kanamycin
Kin gene
drug resistance
