塑料玩具中邻苯二甲酸酯类环境激素检测方法研究

Determination of Phthalates in Plastic Toys by High Performance Liquid Chromatography

文章建立了一种对塑料玩具中5种邻苯二甲酸酯类物质同时分离和检测的高效液相色谱分析法。以shim-pack VP-ODS(4.6mm×150mm,5μm)为分离柱;以甲醇-水(体积比:90:10)为流动相,流速:1.0mL/min,检测波长:230nm。该方法所测得的5种邻苯二甲酸酯类物质在0.5～1000mg/L内具有良好的线性,相关系数r=0.9996,检出限达0.34～0.96μg/L,平均回收率在93.00%～105.23%。在对样品提取和纯化的基础上,对塑料玩具中邻苯二甲酸酯类物质进行了分析,结果令人满意。

High performance liquid chromatography method was developed for rapid separation and determination of 5 kinds of phthalates （phthalic acid esters） in plastic toys. A separation was performed on a shim-pack VP-ODS column with 4.6mm× 150mm, 5μm and CH3OH ： H2O （V/V 90 ： 10） with a flow rate of 1.0mL/min as mobile phase and detection wavelength 230nm. Calibration curve was linear in the concentration range of 0.5-1,000mg/L, LOD 0.34-0.96μg/L and mean recovery ranged from 93.00%-105.23%. The method was used to analyze phthalates in different plastic toys under the optimized conditions of the extraction and purification of samples, with satisfactory results.