化妆品中苯酚和氢醌的气相色谱测定法

Determination of Phenol and Hydroquinone in Cosmetics by Gas Chromatography

目的提出一种用气相色谱FID检测器,以SE-54毛细管柱测定化妆品中苯酚、氢醌的可行性。方法样品经乙醇提取后用具有FID检测器的气相色谱仪分离测定。结果该方法对苯酚和氢醌的检出浓度分别为0.17和0.61μg/g;最低定量浓度分别为0.58和2.02μg/g,方法的重复性(RSD%)为2.01%～2.25%,实际样品的加标回收率苯酚为95.3%～101.4%,氢醌为97.2%～103.6%。结论该方法灵敏度高,精密度和准确度均满足要求,适用于化妆品中苯酚、氢醌的测定。

[ Objective ] To propose a feasible method for determination of phenol and hydroquinone in cosmetics with gas chromatography { GC） FID detector and SE -54 capillary column. [ Methods ] After ethyl alcohol extraction, the sample was separated by GC FID detector. [ Results] The detectable concentration of phenol and hydroquinone by this method was 0.17 and 061 μg/g, respective- ly. The limit of quantification was 0. 58 and 2. 02 μg/g, respectively. The relative standard deviation { RSD% } was 2.01% - 2.25%, the recoveries of phenol and hydroquinone were 95.3% ~ 101.4%, and 97.2% ~ 103.6%, respectively. [ Conclusion]The method is sensitive, with satisfactory precision and accuracy, which is suitable for determination of phenol and hydroquinone in cosmetics.