5-羟色胺对大鼠体液免疫应答的影响

Influence of Serotonin on the Humoral Immune Resnonse in the Rat

本研究用神经药理学方法改变大鼠全身、中枢或外周5-羟色胺(5-HT)的含量,并使用5-HT受体的拮抗剂,观察初次体液免疫应答有无变化,从而探讨5-HT调节体液免疫功能的作用途径和作用机制。结果发现:大鼠腹腔注射5-羟色氨酸(5-HTP)后,体液免疫应答减弱。腹腔注射对氯苯丙氨酸(PCPA)后,免疫应答增强。用去甲丙味嗪(DMI)预处理,然后侧脑室注射5、7-双羟色胺(5、7-DHT);或者单独侧脑室注射5-HT,免疫应答都无显著变化,腹腔注射5-HT后,免疫应答减弱。给予赛庚啶阻断5-HT的受体后,免疫应答增强。本研究结果提示:5-HT对体液免疫应答具有抑制作用;此作用是通过外周途径实现的;其机制可能与免疫细胞上的5-HT受体有关。

The present study investigates the influence of serotonin (5-HT) on the primary humoral immune response to sheep red blood cells (SRBC) in the rat. The results are as follows: Systemic administration of 5-hydroxytryptophaa (S-HTP) resulted in suppression of immune response, parachlorphenylalanine (PCPA) markedly enhanced immune response when injected intraperitoneally. Immune response was significantly affected neither by 5,7-dihydroxytryptamin(5,7-DHT)which was intracerebroventricularly injected into the rat pretreated with desmethylimipramine (DMI)nor by intracerebroven-tricular injection of serotonin. Intraperitoneal administration of serotonin significantly suppressed immune response.Immune function was enhanced following injection of cypro-heptadine, a antagonist of serotoninergic recepeptor. These resulults suggest that sero-ntonin can suppress the primary humoral immune response to SRBC in the rat and im-munomodulation by serotonin is mediated via peripheral mechanism which may be related to the serotoninergic receptor on the immune cells.