流行性出血热患者外周血中性粒细胞内免疫复合物的研究

Study on Immune Complex in Peripheral Polymorphonuclear Leukocyte in Patients of Epidemic Hemorrhagic Fever

本文首次报导了采用双标记免疫荧光技术,从流行性出血热(EHF)患者外周血中性粒细胞(NPL)内检出免疫复合物(IC)。在所检测的18例患者外周血涂片中,均见到NPL内有IC存在,IC呈点状或片状分布于细胞浆中。计数IC^+-NPL(以EHFV-Ag^+-NPL和人IgG^+-NPL表示),发现其检出率因病期而异,在发热期检出率可分别达79-93％和40-70％,随病程进展而下降,至少尿期和恢复期,IC^+-NPL已极少或完全消失。这表明,NPL参与吞噬和清除循环免疫复合物(CIC)的活动,其释出的活性物质和裂解产物又可能造成其后EHF病变过程。

Neutrophilic polymorphonuclear leukocyte(NPL)f rom 18 cases of epidemic hemorrhagic fever(EHF) wac examined for intracellular immune complex(IC) with double staining immunoflorescent assay. IC dispersed as granules or patches were found in the cytoplasm of NPL of all patients.lt showed that the positive rates of the EHFV-Ag+-NPL and human IgG+-NPL varied at different stages of EHF patient.On the febrile-hypotensive stage, EHFV-Ag+-NPL% (79-93%) and human IgG+-NPL% (40-70%)were more than that of the polyuric or convalecent stage. As the disease developed further, IC+-NPL were decreased. At the early convalescent stage, IC+-NPL would hardly be discovered. The difference of IC+-NPL% between the febrile-hypotensive stage and the polyuric or early convalescent stage was significant (P<o.01). It suggests that NPL play an important role in eliminating IC and release other active factors to induce a series of pathological changes.