基于溶胶凝胶法的羊毛织物溶菌酶固定

Immobilization of lysozyme on wool fabric by sol-gel method

为了增强羊毛织物的抗菌性,采用溶胶-凝胶法将溶菌酶固定在羊毛织物上,以期获得良好的抗菌效果。探讨了溶菌酶固定化的主要工艺,得到最佳工艺条件:丙基三甲氧基硅烷(PTMS)与正硅酸甲酯(TMOS)物质的量比为4∶1,水与硅源物质的量比R值为32,溶菌酶质量浓度为25 mg/mL,稳定剂PVA0588质量浓度为2 mg/mL。羊毛织物固载的溶菌酶具有较好的热稳定性和操作稳定性,40℃下保温100 min仍能保持初始酶活的96%,连续使用7次仍能保持初始酶活的52.3%。

Lysozymes were immobilized on wool fabrics by sol-gel method in order to enhance the antibacterial function of wool. The process of immobilization was studied and the optimum parameters were determined, i.e., propyltrimethoxysilane （ PTMS）/tetramethoxysilane （ TMOS ） molar ratio was 4 ： 1, water/silicon source substance volume ratio was 32, lysozyme mass concentration was 25 mg/mL, and PVA 0588 mass concentration was 2 mg/mL. Lysozymes immobilized on wool fabrics presented better thermostability and operation stability compared with free lysozymes. The immobilized lysozyme remained 96 % of the original enzyme activity after an incubation at 40 ℃for 100min, and 52.3 % of the original enzyme activity after seven continuous reuses.