PMA、抗CD3、PHA和A23187对Jurkat细胞表达Tac抗原和高亲和性IL-2受体的调变作用

The Regulatory Effects of PMA, Anti-CDS、 PHA and A23187 on the Expressions of Tac Antigen and High Affinity IL-2 Receptor on Jurkat Cells

本文运用^(125)Ⅰ标记的CD25单克隆抗体和IL-2进行竞争结合试验,发现抗CD3单克隆抗体、PHA和Ca^(++)载体A23187均明显抑制PMA诱导Jurkat细胞Tac抗原的表达。抑制率分别为50.0％、74.5％和87.8％。静止的Jurkat细胞不具有任何亲和性的IL-2结合受体,PMA(20ng/ml)刺激24h后,每个细胞表面表达509个高亲和性IL-2受体,KD值为381PM。

This study analysed the regulotory effects of polyclonal stimuli and Ca++ ionophore on PMA-induced Tac antigen expression on Jurkat cells. It was shown that CDs monoclonal antibody, PHA and A23187 inhibited PMA-induced Tac antigen expression by 50.0%, 74.5% and 87.8%, respectively, by competitive binding assay. The resting Jurkat cells didn't express any high affinity IL- 2 receptor.However, after 24h PMA (20ng/ml) stimulation curkat cells expressed high affinity IL-2 receptors (509 sites/cell, KD 381PM) .