三拗汤及其类方对RSV诱导的哮喘小鼠气道反应性的影响

Effect of San'ao Decoction and its Analogous Prescription on Airway Responsiveness in Asthmatic Mice Induced by Ovaibumin Sensitisation and RSV Infection

目的:观察三拗汤(san'ao Decoction,SAD)及其类方(Analogous Prescription,AP)对呼吸道合胞病毒(Respiratory Syncytial Virus,RSV)诱导的哮喘小鼠气道反应性的影响。方法:Balb/C小鼠70只,随机分为空白对照组、模型组、地塞米松(Dexamethasone,DM)组、SAD组及其类方AP组(分别设大、小剂量),共7组。应用卵清蛋白(Ovalbumin,OVA)多点注射致敏、OVA气道雾化吸入结合RSV滴鼻诱发复制急性病毒性哮喘模型。动物体描箱法测定Ach激发条件下气道反应性(以肺阻力RL表示)并进行肺病理切片。结果:以45、135、405μg·mL-1 Ach浓度激发时,与空白对照组比,模型组RL增高值(与基值比较)均明显升高(P<0.05,P<0.01)。应用药物后各组气道反应性均不同程度降低,尤其类方AP-S组效果更好,能使3个激发浓度的气道反应性均显著降低(P<0.05,P<0.01);药物应用后能减轻支气管周围炎的病变程度。结论:三拗汤及其类方能够降低气道反应性,对RSV诱导的哮喘小鼠有一定的治疗作用;其中类方效果更佳。

In this work, the effect of San＇ao decoction （SAD） and its analogous prescription （AP） on airway responsiveness was studied in asthmatic mice induced by ovalbumin （OVA） sensitization and respiratory syncytial virus （RSV） infection. 70 mice were randomly divided into 7 groups： the PBS control group,the model group, the dexamethasone （DM） group, the SAD-S group,the SAD-L group, the AP-S group, and the AP-L group. An asthma model was established by OVA sensitization with multipoint intraperitoneal injection,followed by repeated inhalation of OVA and repeated intranasal instillation of RSV （1.0×10^6.5 TCID50/mL in 50μL）. Changes in airway responsiveness, which were indicated by lung resistance （RE） stimulated by Ach, were determined and observed by lung histopathology. The results showed that when mice were stimulated by Ach （45,135, 405μg·mL^-1）, RL was significantly increased in the model group （compared with the baseline value） compared with that in the PBS control group（P〈0.05, P〈0.01）. After receiving drugs, rats in five groups, especially in the AP-S group, showed decreased values of increased RL,as well as bronchial inflammation. San＇ao decoction and its analogous prescription may suppress airway hyperresponsiveness, so they have therapeutic effect on asthmatic mice induced by ovalbumin sensitisation and RSV infection, especially the analogous prescription of San＇ao decoction.