黄连素预处理对缺血再灌注损伤大鼠心功能的影响

The influence of Berberine preconditioning on cardiac function of rats with myocardial ischemia-reperfusion injury

目的观察黄连素预处理对缺血再灌注损伤大鼠心功能的影响。方法60只SD大鼠随机分为假手术组?缺血再灌注组?黄连素预处理组和辛伐他汀预处理组,给药组连续腹腔注射7天,假手术组和缺血再灌注组腹腔注射等量生理盐水。在左冠状动脉前降支中上1/3处结扎,1h后剪开结扎线,用止血钳夹闭胸腔,再灌注2h,测量左室收缩压(LVSP),左室舒张末压(LVEDP)及左室最大压力上升及下降速率(±dp/dtmax)。结果与缺血再灌注组比较,黄连素预处理组、辛伐他汀预处理组均能显著增加LVSP(P<0.01),显著减少LVEDP(P<0.01),显著增加±dp/dtmax的绝对值(P<0.01)。黄连素组与辛伐他汀组比较,LVSP和+dp/dtmax明显增加(P<0.05)。结论黄连素预处理能够改善缺血再灌注损伤大鼠的心功能。

Objective To observe the influence of Berberine preconditioning on cardiac function of rats with myocardial ischemia - reperfusion injury. Methods 60 SD rats were randomly divided into 4 groups： Sham - operated group, ischemia - reperfusion group, Berberine group, simvastatin group, then riley had been giving above medicine via peritoneal injection per day for seven days. We ligated them at the middist and urpper 1/3 of anterior descending branch of left eomonary artery, 1 hours later we removed the ligafion and dosed rat＇s chest via hemostat, then gave them reperfusion for 2 hours, and measured LVSP, LVEDP, ± dp/dtmax. Results Compared with ischemia - reperfusion group, the model group of ± dp/dtmax and LVSP of Berbefine group and simvastatin group increased sharply. （ P 〈 0.05 or P 〈 0.01 ）. and the LVEDP of Berberine group and simvastatin group decreased sharply. （P 〈0. 01 ）. Moreover Compared with simvastatin group, the increase of LVSP and ± dp/dtmax of Berberine group is especially obviously （ P 〈 0.01 or P 〈 0.05 ）. Conclusions Berberine preconditioning improved the heart function of rats with myocardial ischemia - reperfusion injury.