细胞芯片制备仪的研制及应用

Development and application of cell microarray instrument

目的研制快速、简单、方便的细胞芯片制备仪,并验证其功能。方法制备仪由相互配合的阵列管固定板和内芯固定板组成,在阵列管固定板上按行列方式设有多根带微孔的阵列管,在内芯固定板上设有多根与阵列管固定板上各阵列管对位配合的阵列管内芯。工作中通过阵列管及内芯间形成的空腔结构,将细胞样本通过琼脂糖包埋于空腔内,使之在带微孔的阵列管空间进行脱水、透明、浸蜡等处理,进而制成细胞芯片石蜡包埋块,再经过切片、染色后即可完成细胞芯片的制备。结果细胞芯片制备仪能够迅速制备出27种细胞的细胞芯片,肉眼观点阵完整,分布均一,经HE切片及免疫组化染色证实,染色效果良好,结果可靠。结论细胞芯片制备仪构造简单,操作方便,能够迅速成型制备出多种不同细胞类型的细胞芯片,适用于推广使用。

Aim To develop the cell microarray of fast, simple and convenient and verify its function. Methods Preparation of instrument by the interaction of the array of tubes fixed plate and fixed plate inner core composed of a fixed plate in the array of tubes comprises a number of ways according to the ranks of the root zone of the array of micro-tubes, including the core fixed-board which comprises a tubes of various array of alignment with the core tube. Work number of roots and an array of fixed-board array of through the array of tubes and the cavity formed between the inner core structure of the cell sample by agarose embedded in the cavity so that the array of tubes with a pore space dehydration, transparent, dipped wax treatment, resulting in the manufacture cell microarray paraffin- embedded blocks, and then after the sectioned and stained immediately, completion of cell microarray. Results Cell chips from 27 kinds of cells can be quickly prepared by this cell chips instrument, the cell chip array is complete and uniform distribution, the cell microarray by HE sections and immunohistochemical staining confirmed that the staining result is good and reliable. Conclusion The cell chip instrument possesed simply structure, easy operation, can be prepared quickly and is suit to use and promote.