淋巴瘤细胞膜P糖蛋白、信号转导与转录激活因子3及bcl-2表达水平与化疗耐药的相关性

Correlation of P-gp, bcl-2, STAT3 and the resistance of lymphoma cells to chemotherapeutic agents

目的探讨淋巴瘤细胞膜P糖蛋白（P—gP）、细胞胞内信号转导与转录激活因子3（STAT3）、bcl-2、白细胞介素（IL）-6及IL-10表达水平与淋巴瘤患者化疗耐药的相关性。方法对疑诊淋巴瘤的18例患者，手术活检淋巴结，应用流式细胞术（FCM）测定细胞膜P—gP、胞内STAT3、抗凋亡蛋白bcl-2、细胞因子IL-6及IL-10表达水平，前瞻性研究与化疗疗效的关系。其中，化疗耐药组10例，化疗敏感组8例，以10例炎性淋巴结增生为正常对照。结果化疗耐药组P—gP和bcl-2表达水平均高于化疗敏感组（P=0．01和P=0．039），而STAT3、IL-6及IL-10表达水平差异无统计学意义（P〉0．05）。淋巴瘤细胞膜P—gP表达水平高于炎性淋巴结增生（P=0．01），STAT3表达水平明显低于炎性淋巴结增生（P=0．04），淋巴瘤与炎性淋巴结增生bcl-2、IL-6及IL-10表达水平差异无统计学意义（P〉0．05）。结论bcl-2、P—gP表达水平的高低与恶性淋巴瘤化疗疗效密切相关，STAT3参与了淋巴瘤细胞的信号转导，是否参与了多药耐药信号转导尚不能肯定，而胞内IL-6、IL-10的表达与化疗疗效未见相关。

Objective To investigate the correlation of P-gp, bcl-2, STAT3, IL-6, IL-10 and the resistance lymphoma cells to chemotherapeutic agents. Methods There were 28 patients, among them were divided into three groups： refractory lymphoma, lymphoma which is sensitive to agents and lymphodenitis. Pglyeoprotein （P-gp） on lymphoma cell membrane, STAT3, bel-2, IL-6 and IL-10 level into lymphoma cell were detected using FCM, and the correlation between them and chemotherapy efficacy were analysed. Results In refractory lymphoma patients, P-gp and bcl-2 are significant higher than that of the group which is sensitive to agents （ P =0.01,P =0.039）, but STAT3, IL-6 and IL-10 were not significant different between these two groups （P 〉0.05）. P-gp is significant higher in lymphoma than in lymphodenitis （P =0.01）. STAT3 in lymphoma is significant lower than that of in lymphodenitis （P =0.04）. The level bcl-2, IL-6 and IL-10 between lymphoma and lymphodenitis are not significant different（P 〉0.05）. Conclusion The expression level of bcl-2 and P-gp is correlated to resistance to the chemotherapeutic agents in lymphoma cells. STAT3 play a role in lymphoma cell signal transduetion, but it is not certain in lymphoma cell muhidrug resistance.