摘要
目的探讨淋巴瘤细胞膜P糖蛋白(P—gP)、细胞胞内信号转导与转录激活因子3(STAT3)、bcl-2、白细胞介素(IL)-6及IL-10表达水平与淋巴瘤患者化疗耐药的相关性。方法对疑诊淋巴瘤的18例患者,手术活检淋巴结,应用流式细胞术(FCM)测定细胞膜P—gP、胞内STAT3、抗凋亡蛋白bcl-2、细胞因子IL-6及IL-10表达水平,前瞻性研究与化疗疗效的关系。其中,化疗耐药组10例,化疗敏感组8例,以10例炎性淋巴结增生为正常对照。结果化疗耐药组P—gP和bcl-2表达水平均高于化疗敏感组(P=0.01和P=0.039),而STAT3、IL-6及IL-10表达水平差异无统计学意义(P〉0.05)。淋巴瘤细胞膜P—gP表达水平高于炎性淋巴结增生(P=0.01),STAT3表达水平明显低于炎性淋巴结增生(P=0.04),淋巴瘤与炎性淋巴结增生bcl-2、IL-6及IL-10表达水平差异无统计学意义(P〉0.05)。结论bcl-2、P—gP表达水平的高低与恶性淋巴瘤化疗疗效密切相关,STAT3参与了淋巴瘤细胞的信号转导,是否参与了多药耐药信号转导尚不能肯定,而胞内IL-6、IL-10的表达与化疗疗效未见相关。
Objective To investigate the correlation of P-gp, bcl-2, STAT3, IL-6, IL-10 and the resistance lymphoma cells to chemotherapeutic agents. Methods There were 28 patients, among them were divided into three groups: refractory lymphoma, lymphoma which is sensitive to agents and lymphodenitis. Pglyeoprotein (P-gp) on lymphoma cell membrane, STAT3, bel-2, IL-6 and IL-10 level into lymphoma cell were detected using FCM, and the correlation between them and chemotherapy efficacy were analysed. Results In refractory lymphoma patients, P-gp and bcl-2 are significant higher than that of the group which is sensitive to agents ( P =0.01,P =0.039), but STAT3, IL-6 and IL-10 were not significant different between these two groups (P 〉0.05). P-gp is significant higher in lymphoma than in lymphodenitis (P =0.01). STAT3 in lymphoma is significant lower than that of in lymphodenitis (P =0.04). The level bcl-2, IL-6 and IL-10 between lymphoma and lymphodenitis are not significant different(P 〉0.05). Conclusion The expression level of bcl-2 and P-gp is correlated to resistance to the chemotherapeutic agents in lymphoma cells. STAT3 play a role in lymphoma cell signal transduetion, but it is not certain in lymphoma cell muhidrug resistance.
出处
《白血病.淋巴瘤》
CAS
2009年第12期732-734,共3页
Journal of Leukemia & Lymphoma
基金
山西省卫生厅科技招标项目(200611)