流式细胞仪用于荧光标记糖皮质激素受体的淋巴细胞研究

Determination of Glucocorticoid Receptors in Human Peripheral Lymphocytes with Flowcytometer and Fluorescein Labeled Ligands

本文应用糖皮质激素受体(GR)的荧光素标记配体(DF)和流式细胞仪对正常人外周血淋巴细胞内的GR进行了研究。实验采用全血法和淋巴细胞分离法制备淋巴细胞,用相同的实验条件和信息处理进行比较,发现二种不同方法制备淋巴细胞对GR测定无明显影响。正常人外周血淋巴细胞阳性率为14.457±5.975。不同性别的GR淋巴细胞阳性率分别为,男性:18.128±6.3;女性11.058±2.984,有统计意义上的差别。

We investigated the distribution of glucocorticoid receptors (GR) in human peripheral lymphocytes with flowcytometer and fluorescein labeled ligands (DP). Hemolytic method as well as Ficoll Hypaque solution were used for the preparation of lymphocytes . With the same test conditions and measuring parameters, the results indicated that there were no difference between the two methods in GR assay. The percentage of GR positive cells in normal human preipheral lymphocytes is 14.457±5.957,with male:18.128±6.3 and female:11.085±2.984. The difference is significant.