当归中阿魏酸的超临界CO_2萃取工艺条件及GC-MS分析

Study on the extraction-process of ferulic acid from Angelica sinensis (Oliv.) Diels with SFE-CO_2 and components analyzing by GC-MS

考察超临界CO2萃取当归中阿魏酸的最佳工艺条件、有效物质定量方法及萃取物组成。采用正交试验法,使用乙醇为夹带剂,以收膏率和提取物中阿魏酸含量为目标函数,对药材粒度、夹带剂(乙醇)浓度、萃取压力、萃取温度等当归超临界萃取工艺条件进行研究,GC-MS分析和比较夹带剂对萃取效果的影响。结果优选出最佳超临界萃取条件是:药材粒度0.30～0.85 mm、夹带剂(乙醇)浓度80%、萃取压力35 MPa、萃取温度60℃-50℃-45℃;纯CO2超临界萃取物鉴定出62个组分,使用夹带剂的超临界萃取物鉴定出83个组分,两者有54个相同组分;优选超临界萃取工艺所得的挥发油及阿魏酸提取量较高,使用夹带剂有利于阿魏酸等极性物质的萃取。

The supercritical CO2 extraction-process of ferulic acid from Angelica were optimized in this study, and the components of extracts were analyzed by Gas Chromatography mass spectrometry （GC-MS）. The yield of volatile oil and ferulic acid was regarded as the markers and the content of ferulic acid was determined by HPLC. The extraction processing conditions were optimized by orthogonal test. The extraction effects were compared by GC-MS analysis on co-solvent were employed or not. The optimized extraction technique was： the particle size was between 0.30-0.85 ram, the extraction pressure was 35 Mpa, and the program of extraction temperature was 60℃-50℃-45℃, 80% C2H5OH as entrainer. Sixty-two components were identified in the pure CO2 extracts, eithty three components in SFE-CO2 extracts, and fifty-four components in both of them. The optimal process feasible with the advantages of high production of volatile oil and ferulic acid, The employ of co-solvent can increase the content of ferulic acid in the extracts.