翦股颖属植物基因组DNA提取方法的比较

Comparison of DNA extraction methods of Agrostis genome

采用试剂盒法、高盐低pH法、改良高盐低pH法、吸附柱法、SDS法、改良CTAB法,分别对翦股颖属植物品种Penncross、Seaside、PennA-4、粤选1号和新品系粤选10号的基因组DNA进行提取并经DNA电泳检测、蛋白分析仪分析和RAPD分析。结果表明:改良的CTAB法、改良高盐低pH法能够分离出产率高、纯度高、质量好的DNA,而吸附柱法的DNA产率偏低、但纯度较高,3种方法均能扩增出清晰的条带。

Six methods included kit, high salt low pH, modified high salt low pH, column adsorption, SDS （Sodium dodecyl sulfate）, modified CTAB （cetyltrimethyle ammonium bromide） were compared for extracting DNA from Agrostis varieties： Penncross, Seaside, PennA-4, Yuexuan No.1 and new strain Yuexuan No.10. The results showed that modified high salt low pH, modified CTAB and column adsorption methods could extract DNA of high-quality and high-integrity, as determined by agarose gel electrophoresis and UV-spectroscope. While the DNA productivity of column adsorption methods was lower than other two methods. The results of RAPD indicated that DNA extracted by these three methods satisfied the standard for gene molecular biological experiments.