PCR检测降钙素基因高甲基化在白血病微小残留病灶诊断中的价值

THE VALUE IN DIAGNOSING MINIMAL RESIDUAL DISEASE IN LEUKEMIA BY USING POLYMERASES CHAIN REACTION TO DETECT HYPERMETHYLATION IN THE CALCITONIN GENE

为探讨甲基化分析在白血病微小残留病灶（MRD）中的价值，用酶切加PCR技术研究31的初诊期急性髓细胞白血病（AML），14例对照者以及在18个月中动态观察5例AML治疗前后降钙素（CT）基因高甲基化的变化。31N初诊期AML中25例，14例对照者中的0呈现CT基因高甲基化（P＜0．01）。动态观察的5例AML，全部初诊期及完全缓解时的4例呈现CT基因高甲基化，其中3例分别于完全缓解后3个月、5个月、6个月复发。完全缓解和CT基因高甲基化阳性1例及阴性1例未复发。提示该方法有助于检测AML的MRD。

The relapse of leukemia was resulted from surviving minimal residual disease (MRD) after primary treatment, so it is clinically important to detect MRD. The author combined the endonuclease digestion with polymerases chain reaction to study hypermethylation in the calcitonin (CT) gene of 31 cases with acute myelogenous leukemia (AML) at dignosis. A control group was consisted of 14 individuals and 5 cases with AML before/after treatment during 18 months. It was found that 25 out of 31 cases with AML at diagnosis, none of the control group showed CT gene 'hypermethylation (P<0. 01). Meanwhile, 4 out of 5 cases with AML who showed CT gene hypermethylation at diagnosis exhibited CT gene hypermethylation during complete remission, 3 of which relapsed after 3, 5, 6 months, respectively. However, 2 cases with AML during complete remission, one of them showed CT gene hypermethylation, the other haven't relapsed so far. The results suggested that the method be useful to detect MRD in AML.