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低强度超声对小鼠肝癌H_(22)细胞抑制效应的初步研究 被引量：1

Inhibition of low-intensity ultrasound radiation on hepatocellular carcinomas cell line H_(22) cells
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摘要 目的:观察低强度超声对小鼠肝癌H22细胞体外生长的影响。方法:以低强度超声辐照小鼠肝癌H22细胞,采用倒置显微镜观察和MTT法检测观察超声对小鼠肝癌H22细胞的抑制作用。结果:低强度超声波能够显著抑制小鼠肝癌H22细胞的增殖,并且跟辐照时间呈剂量依赖关系。辐照后24h其MTT光吸收值随着超声作用时间的延长而显著降低。结论:低频超声辐照对小鼠肝癌H22细胞体外生长具有明显的抑制作用。 Objective：To investigate the inhibition effect of low-intensity ultrasound radiation on hepatocellular carcinomas cell line H22 cells.Methods： The growth inhibitory effect of ultrasound exposure（1.7MHz,1.35W/cm2）on H22 cells was observed using light microscopy and MTT colormetric assay.Results： The absorbance value of MTT significantly decreased at 24h after ultrasound radiation as compared to the control.Conclusion ： Ultrasound radiatiion could obviously inhibit the proliferation of H22 cells.
作者 徐静 许川山 夏新蜀 王昕娜 王萍 向君彦
机构地区 重庆医科大学超声影像学研究所 重庆医科大学附属第二医院康复理疗中心 重庆医科大学附属第一医院康复医学科
出处 《激光杂志》 CAS CSCD 北大核心 2009年第6期69-69,71,共2页 Laser Journal
基金 国家高技术研究发展计划(863计划2006AA0224F0)资助
关键词 低强度超声 细胞抑制 小鼠肝癌H22细胞 ultrasound radiation hepatocellular carcinomas inhibition
分类号 R477 [医药卫生—护理学]
  • 相关文献

参考文献11

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共引文献4

同被引文献10

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激光杂志
2009年 第6期

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