马病毒性动脉炎诊断技术研究进展

Progress on Diagnostic Technology for Equine arteritis virus

马病毒性动脉炎(EVA)是马属动物之间通过呼吸道或生殖器官传播的传染病,是危害养马业及赛马比赛的重要疾病之一,应用各种诊断技术对该病做出准确的诊断是预防和控制该病的前提。EVA的诊断技术包括检测病毒,检测血清抗体,涉及的技术和方法包括病毒的分离及中和试验、血凝及血凝抑制试验、免疫荧光试验以及酶联免疫吸附试验等。每种诊断技术都有其各自的优势和局限性,应根据目的及工作条件等综合情况予以选择,论文就各种诊断技术的原理、方法及特点进行了概述和比较,并对今后的应用前景和发展趋势进行了展望。

Equine viral arteritis is an infectious disease of Equus animals, which transmitted through respiratory tract or reproductive tract ,and is one of important diseases of horses. The application of all kinds of diagnostic techniques for the accurate diagnosis of the disease is the premise for the prevention and the con- trol this disease. The EVA diagnostic techniques include detecting virus, serum antibodies. The technologies involved include virus isolation,the neutralization test, hemagglutination and hemagglutination inhibition test,immunofluorescence assay and enzyme-linked immunosorbent assay,and so on. Each technique has its respective superiority and limitation,and should be chosen based on the purpose and conditions. The paper reviewed and compared the principles and characteristics of different dignostie techniques,and analyzed the application prospect and trend of these techniques.