摘要
目的:研究薤白中总皂苷纯化工艺。方法:采用大孔吸附树脂对薤白总皂苷进行吸附纯化,以总皂苷的收率、质量分数为考察指标综合评价。结果:薤白总皂苷纯化工艺为提取液上D101大孔树脂柱,吸附3h,以水和5%乙醇洗至无色,再以60%乙醇洗脱,60%乙醇部分为皂苷部位。结论:确定了薤白中皂苷部位的提取纯化工艺,总皂苷含量达到55%。
Objective： To find the best purification process of total saponin from Allium macrostemon Bunge. Methods： The content and weight of total saponin were used as markers to screen the best method of extraction and adsorption conditions of macroporous adsorption resin. Results： The best purification process was as follows： the extracts were adsorbed by D101 macroporous resin for 3 h and eluted by water, 5% and 60% ethanol. 60% ethanol fractions were saponin fraction. Conclusions： We found the best purification process of total saponin from Allium macrostemon Bunge. The content of total saponin were over 55%.
出处
《药学与临床研究》
2009年第6期455-458,共4页
Pharmaceutical and Clinical Research
基金
江苏省科技支撑计划-社会发展课题项目(编号:BE2008644)
关键词
薤白
皂苷
大孔树脂
Allium macrostemon Bunge.
Saponin
Macroporous adsorption resin.
