摘要
脲酶是一种来源广泛的植物蛋白酶,能高效、专一的催化尿素分解。本文利用双醛淀粉(DAS)作为酶固定化载体对脲酶进行固定化。同时研究反应温度,反应时间,pH,载体的醛基含量,载体与酶的配比对固定化酶活性的影响。经实验确定,最佳工艺条件为:双醛淀粉载体醛基含量80%,反应pH值6.0,反应温度25℃,反应时间25h,酶与载体的配比为1:1(mL/g)
As one kind of origin widespread plant protemase, the urease can selectively catalytic decomposition of urea with high effect. In this paper, dialdehyde starches were used as carrier for immobilization of urease. The effects of temperature, time, pH, aldehyde group content of carriers and carrier/enzyme ratio on immobilizing urease were studied. The best conditions were determined as follows： 80% of aldehyde in dialdehyde starch, 6.0 of the reaction pH value, 25℃ of reaction temperature, 25h of reaction time, and the enzyme-cartier ratio of 1：1 （mL / g）
出处
《现代食品科技》
EI
CAS
2009年第12期1448-1450,1453,共4页
Modern Food Science and Technology
基金
高等学校博士学科点专项科研基金(20070561078)
广东省产学研结合项目(2007B090400003)
广东省科技攻关项目(2008B021100016
2005B20401006)
关键词
脲酶
固定化
载体
双醛淀粉
urease
immobilized
carriers
dialdehyde starch
