不同解冻方法对辽宁绒山羊精子活力的影响

Study on Effect of Different Thawing Ways on Activity of Frozen Semen Straws in Liaoning Cashmere Goat

通过比较三种精液冷冻保存稀释液配方(A、B、C)对山羊细管冷冻精解冻后精子活力的影响,结果表明,三种稀释液配方(A、B、C),冷冻-解冻后精子活力有极显著的差异(P<0.01),分别为42.57%、37.34%、33.03%,A显著优于B液和C液.而且用不同的水浴温度和解冻时间进行解冻,通过精子活力的比较,寻找出最佳的解冻温度为38℃,解冻时间为60s.

The aim of study is comparing the formula diluent of the three semen （A,B,C）,and to do detecting of the active.The result indicates that A ,B,C diluent ingredient the active of semen are average 42.57% ,37.34% ,33.03%,A beyond B and C ,there is a significant different and very significant different （P 〈0.01 ）.However the temperature and time of defrosting are 38℃, 60s.