中药佛手散对孕鼠妊娠结局的影响及机理研究

The Effect of Foshou Powder on the Pregnancy Outcome of Normal Pregnant Rat

目的:探讨中药佛手散对正常大鼠妊娠结局的影响及机理,为临床孕妇活血药物的运用提供依据。方法:将日龄为42～56dSPF级Wistar大鼠雌雄配对后筛选40只孕鼠,分为对照组及低、中、高剂量4组,各10只,统一给药灌胃,每天1次,持续7d,观察妊娠情况,于妊娠第18～19天处死大鼠,解剖后立即数子宫着床点数以及活胎数,计量胎盘重量、子宫重量。各组大鼠开胸心脏采血,测量其全血黏度、血浆黏度等,并采用放射免疫法测定血清及子宫、胎盘组织匀浆液肿瘤坏死因子α浓度。结果:各组孕鼠子宫重量和平均胎盘重量无显著差异,正常妊娠对照组血液黏度高于其他组,中剂量组、高剂量组流产率、血清/子宫胎盘组织中肿瘤坏死因子α高于其它组。结论:佛手散可以改善妊娠大鼠的血液黏滞程度,低剂量运用对正常妊娠大鼠的妊娠结局无不良影响,当剂量超过一定限度时,则会升高血清及局部组织的肿瘤坏死因子α,使正常妊娠大鼠自然流产。

Objective：To investigate the effect of Foshou powder on the pregnancy outcome of normal pregnant rat in order to provide the clinical basis for application of blood-activiting traditional Chinese medicine in the pregnant women. Methods： The female and male Wistar rats aged 42 to 56 days were mated. Forty pregnant rats were selected and equally assigned to 4 groups： the control group,the low-dose group,the moderate-dose group and the high-dose group. They received intragastric administration of Foshou powder per day for 7 consecutive days. On the day 18 or 19 of pregnancy,the pregnant rats were sacrificed. The numbers of blastocyst implantation sites and living fetus as well as the weight of placenta and uterus were evaluated. Whole blood viscosity,plasma viscosity and the concentration of tumor necrosis factor （TNF）-α in serum and the tissue homogenized of uterus and placenta were measured. Result：There were no significant differences in the average weight of uterus and placenta among the pregnant rats in the 4 groups. The blood viscosity in the control group was higher than those of the other three groups （ P 0.05）. Both the rates of abortion and the concentration of TNF-α in serum and the tissue homogenized of uterus and placenta were higher in the moderate-dose group and the low-dose group than those in the other two groups（ P 0.05）. Conclusion： The Foshou powder could improve the hypercoagulable state of pregnancy rat. The low dose of powder have no adverse effects on pregnancy rat. However,when the dose exceeds certain limit,the concentration of TNF-α in serum and local tissue could be evaluated,resulting in miscarriage in pregnant rats.