AMP和MH对菊花绿蕾期叶片叶绿体Ca^(2+)-ATPase、Mg^(2+)-ATPase及超微结构的影响

Effect of AMP and MH on Ca^(2+)-ATPase,Mg^(2+)-ATPase and Ultrastructure in Chloroplast of Chrysanthemum Leaves in Green Bud Stage

在秋菊姹紫嫣红品种的营养生长期,以氨卞西林钠(AMP,1 000mg/L)和不同浓度马来酰肼(MH 100mg/L、200mg/L和300mg/L)的水溶液进行叶面喷雾处理,初步研究了AMP和MH对菊花绿蕾期叶片光合速率、叶绿体Ca2+-ATPase、Mg2+-ATPase活性和叶绿体超微结构的影响。结果表明,AMP处理使菊花绿蕾期叶片上述生理指标均明显高于对照,叶绿体片层结构数量和密集程度明显增加,嗜锇颗粒减少。MH处理叶绿体Ca2+-ATPase和Mg2+-ATPase活性比对照明显降低,且随处理升高呈先降后升的变化趋势;在100~200mg/L MH浓度范围内,光合速率和叶绿体片层结构数量和密集程度均高于对照,嗜锇颗粒减少,且处理效应随处理浓度升高而有所减弱,在300mg/L处理下,光合速率低于对照且叶绿体片层结构趋于解体,嗜锇颗粒数量明显增多。

In the vegetable growing stage of chrysanthemum Chazhiyanhong, 1 000mg/L of Ampicillin （AMP） and different concentrations （100mg/L,200mg/L and 300mg/L）of Maleic hydrazide （MH） were sprayed to study the effects of them on the photosynthesis rate, Ca^2＋-ATPase, Mg^2＋-ATPase and ultrastructure in chloroplast of the leaves in the green bud stage,and the time of flowering. The results showed that：under the treatment of AMP the physiological index above mentioned,the mumber and the density of chloroplast lamellae increased, and the osmiophilic globules in chloroplast decreased. Under the treatments of MH the activities of Ca^2＋-ATPase and Mg^2＋-ATPase were lower than CK which decreased early and then increased later. Moreover,at the treatments of 100-200mg/L MH, the photosynthesis rate, the number and the density of chloroplast lamellae were higher with less osmiophilic globules in chloroplast,and were negative correlated;as long as the 300mg/L MH treatment was concerned, it was the reverse.