摘要
DR1709 is a predicted Mn2+ transporter in Deinococcus radiodurans(D.radiodurans). The mensuration method to evaluate protein viability with two-dimensional electrophoresis in D.radiodurans and the mutants was established in this study. The results showed that after DR1709 was disrupted, the expressions of DR1120 (acetokinase), DR1691 (heat shock protein), DR1485 (putative lipase), DR2095 (putative c-type cytochrome) and other three hypothetical proteins (DR0124, DR0047 and DR2474) were repressed. However the expression of DR1794 (putative nosX) was induced. Phenomena above suggested that the increased radiation-sensitivity of the mutant cells may be attributed to not only the protection of gene DR1709, but also the proteins' different expressions between the wild type and the mutant might also play important roles in protecting D.radiodurans from irradiation. Although DR2095 was a homologue of c-type cytochrome, it has no realitic functions.
DR1709 is a predicted Mn^2＋ transporter in Deinococcus radiodurans（D.radiodurans）. The mensuration method to evaluate protein viability with two-dimensional electrophoresis in D.radiodurans and the mutants was established in this study. The results showed that after DR1709 was disrupted, the expressions of DRll20 （acetokinase）, DR1691 （heat shock protein）, DR1485 （putative lipase）, DR2095 （putative c-type cytochrome） and other three hypothetical proteins （DR0124, DR0047 and DR2474） were repressed. However the expression of DR1794 （putative nosX） was induced. Phenomena above suggested that the increased radiation-sensitivity of the mutant cells may be attributed to not only the protection of gene DR1709, but also the proteins＇ different expressions between the wild type and the mutant might also play important roles in protecting D.radiodurans from irradiation. Although DR2095 was a homologue of c-type cytochrome, it has no realitic functions.
基金
Supported by National Project 973 (No. 2004CB719604)
