基于分子印迹膜的电导型传感器检测牛乳中琥珀酸氯霉素残留

A Conductometric Sensor for Determination of HS-CAP in Milk Based on Molecularly Imprinted Films

采用光聚合法在丝网印刷电极表面原位聚合制备出含有琥珀酸氯霉素(HS-CAP)分子印迹位点的分子印迹膜。通过扫描电镜(SEM)分析了该膜的表征。将修饰有分子印迹膜的丝网印刷电极与电导分析仪相连接,组装成检测HS-CAP残留的电导型传感器,利用该传感器检测建立了检测HS-CAP的标准曲线并对实际牛奶样品进行检测。结果表明,传感器对HS-CAP分子具有良好的特异性识别能力,检出限达0.05 mg·L^(-1),线性范围0.2～1.0 mg·L^(-1),样品检测回收率介于94.3%～98.5%之间。

Molecularly imprinted films （MIF） with specific recognition sites for Chloramphenicol succinate （HS-CAP） were prepared on screen printed electrodes by photopolymerisation. The superficial characteristic of the MIF was investigated using scanning electron microscope （SEM）. A conductometric sensor system for detecting HS-CAP was constructed by incorporating the screen printed electrodes covered with MIF into electrode slots of a conductometer. Calibration curve for HS-CAP was established and determination of HS-CAP in milk samples was conducted, using the conductometric sensor system. The sensors based on MIF showed high selectivity to HS-CAP and the response of MIF-sensors to concentration of HS-CAP displayed a linear correlation over a range from 0.2 mg·L^-1 to 1.0 mg·L^-1 with a detection limit of 0.05 mg·L^-1. The recoveries of 94.3%-98.5% were obtained for HS-CAP in milk samples.