添加金属离子对玉米发芽过程中酶活力影响的研究

Study on Adding Metal Ions to Increase the Activity of Enzyme Systems During Corn Germination

在玉米发芽过程中通过浸泡方式添加K+、Cu2+、Mg2+、Zn2+、Ca2+来提高玉米植酸酶、淀粉酶和蛋白酶的活力,使以玉米为主要原料的饲料不需再外加植酸酶等酶制剂,降低饲料生产成本。实验发现,Ca2+对植酸酶的影响最为明显,最大酶活力达到5.857 U/g(干玉米),为对照的2.5倍;对淀粉酶和蛋白酶也有影响,α-淀粉酶、β-淀粉酶和蛋白酶的最大酶活力分别为对照的4.4、1.5、2.6倍。

To increase the activity of phytase, amylase and protease in the corn enzyme, add the metal ions K^＋, Cu^2＋, Mg^2＋, Zn^2＋ and Ca^2＋ on corn germination through the way of soaking, in order to make the feed which supply by the corn as the main raw materials do not need to add enzyme preparation such as phytase, and the production costas will be reduced. It was found that phytase was impacted by Ca^2＋ most obviously, the greatest activity achieved 5.857 U/g（dry）, an increase of 2.5 times compared to the blank, and for the protease and amylase also had an impact, the greatest activity of or-amylase, 15-amylase and protease were 4.4 times, 1.5 times and 2.6 times compared to the blank.