白介素29体外抗乙肝病毒作用

The antiviral effect of interleukin 29 against hepatitis B virus in vitro

目的研究白介素29(IL-29)体外抗乙肝病毒的效果。方法从RNA水平探讨了IL-29抗乙肝病毒的效果。RT-PCR分析IL-29诱导抗病毒蛋白MxA、2′,5-′OAS、PKR和RNase L mRNA水平表达;同时Western blot分析IL-29激活的信号通路以探讨其抗乙肝病毒的机理。结果IL-29能明显降低HepG2.2.15细胞中乙肝病毒mRNA的水平,且能够上调抗病毒蛋白MxA和2′,5-′OAS的mRNA表达并激活ERK1/2、AKT信号途径。结论在HepG2.2.15细胞中IL-29有显著的抗乙肝病毒作用。

Purpose To explore the antiviral effect of interleukin 29（IL-29） on hepatitis B virus in vitro.Methods To study the antiviral effect of IL-29 against hepatitis B virus by the amount of HBV mRNA detected.Through the quantity of mRNA translated from genes of MxA,2′,5′-OAS,PKR and RNase L as well as the signal pathway induced by IL-29,we used RT-PCR and Western blot to discuss the anti-hepatitis B virus mechanism which was stimulated by IL-29.Results The amount of HBV mRNA in HepG2.2.15 cells was reduced by stimulation of IL-29. The expression of MxA and 2′ ,5′-OAS was up-regulated, as well as P-ERK and P-AKTwere activated by IL-29. Conclusion These findings showed that IL-29 had obvious antiviral activity towards HBV in HepG2.2.15 cells.