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TCF2/HNF1β基因多态性与江苏地区人群T2DM的相关性 被引量：1

Association of TCF2/HNF1β gene polymorphism with type 2 diabetes in Jiangsu area
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摘要 目的观察肝细胞核因子TCF2/HNF1β基因多态性(TCF2-SNP4 rs1016991)分布及其与江苏地区人群2型糖尿病(T2DM)的相关性。方法对198名T2DM和105名正常糖耐量者的TCF2-SNP4 rs1016991进行检测,HOMA-IR估测外周组织胰岛素敏感性,并测定其他糖脂代谢相关临床指标,凝胶阻滞电泳分析TCF2-SNP4 rs101699(T→A)对DNA蛋白结合能力的影响。结果T2DM组中T/T基因型频率及T等位基因频率均明显高于对照组(P<0.05);T2DM组中T/T基因型研究对象的HOMA-IR指数明显高于A/A基因型(P<0.05);分别含有(T/A)DNA探针结合序列在DNA蛋白结合能力有明显差异。结论TCF2-SNP4 rs1016991与江苏地区人群T2DM及胰岛素敏感性具有相关性。 Objective To investigate the distribution of TCF2/HNF1β gene polymorphism （TCF2-SNP4 rs1016991 ） in type 2 diabetic patients in Jiangsu area. Methods The genotype （TCF2-SNP41） was determined by method of direct sequencing for presence of the polymorphism in 303 subjects, including 198 subjects with newly-diagnosed type 2 diabetes （T2DM） and 105 with normal glucose tderance （NGT）. The tissue insulin sensitivity was assessed by using formula of ho- meostasis model assessment （HOMA-IR） in T2DM group. The influence of TCF2-SNP4 （T→A）on DNA binding ability was tested by using gel shift assay. Results In T2DM group, the TT genotype frequency and T allele frequency was significantly higher than in NGT group （ P 〈 0.05 ） and the TT genotype had a higher HOMA-IR than those with AA genotype （ P 〈 0.05 ）. Gel shift assay showed different DNA-binding ability of DNA probes containing TCF2-SNP4 （T/A）. Conclusion TCF2- SNP4 rs1016991 may be associated with T2DM and insulin sensitivity in Jiangsu area nationality.
作者 俞力 施良 张海明 王莹 王东 邓梅香 陈桂英 陈军建
机构地区 江苏大学附属医院 日本岐阜大学医学研究科
出处 《山东医药》 CAS 北大核心 2009年第48期26-28,共3页 Shandong Medical Journal
基金 江苏省镇江市社会发展科技计划(SH2006020)
关键词 肝细胞因子1β 多态性 糖尿病 非胰岛素依赖型 TCF2/HNF1β polymorphism diabetes mellitus, non-insulin dependent
分类号 R587.1 [医药卫生—内分泌]
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参考文献8

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山东医药
2009年 第48期

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