黄毛耳草药材的薄层色谱研究

TLC Study of Hedyotis Chrysotricha

目的:建立黄毛耳草药材的定性控制方法。方法:采用薄层色谱法分别检查药材中的环烯醚萜类、黄酮类和内酯类成分。结果:环烯醚萜类、黄酮类和内酯类成分分别在以氯仿-乙酸乙酯-甲醇-甲酸(5∶4∶2∶0.5)、氯仿-乙酸乙酯-甲醇-甲酸(5∶1∶1∶0.5)和氯仿-乙酸乙酯-甲酸(5∶3∶1)为展开剂的条件下获得理想分离。结论:该方法简便、快速,可作为黄毛耳草药材的定性控制方法。

Objective： Establish the qualitative control method of Hedyotis chrysotricha. Methods ： We use the the thin layer chromatography method to detect the flavanone class, lactone and iridoids class ingredients. Results： These three kinds of ingredients are all seperated very well by the Chloroform - ethyl acetate - methanol - formic acid （5 ： 4 ： 2 ： 0.5 ）, Chloroform - ethyl acetate - methanol - formic acid （5 ： 1 ： 1 ： 0.5 ）, Chloroform - ethyl acetate - formic acid （5 ： 3 ： 1 ）. Conclusion ：This method is simple, quick,and is applicable to the quality control of Hedyotis chrysotricha.