细辛药材不同部位GC指纹谱分析及药材含量测定

Study on GC Fingerprint and Assay of Herba Asari in Different Parts

目的：建立细辛药材不同部位GC指纹图谱及α-蒎烯、1、8-桉叶素、甲基丁香酚3种成分同时测定的定量分析方法。方法：利用毛细管气相色谱法对采集的6批细辛药材地上部分、根及根茎进行指纹谱分析;采用外标一点法,同时测定了来源于我国8个省34批细辛商品药材中α-蒎烯、1、8-桉叶素、甲基丁香酚含量。结果：以39个共有峰为指标,建立了细辛不同部位气相色谱指纹谱。GC法测定α-蒎烯、1、8-桉叶素、甲基丁香酚含量,进样量在2.58~46.44ng、2.292~32.088ng、3.7768~151.872ng范围内呈良好的线性关系,回归系数分别为0.9990、0.9990、0.9997,平均回收率为95.35%（RSD=2.38%）、96.11%（RSD=3.08%）、94.89%（RSD=2.71%）。结论：GC法建立的细辛药材不同部位指纹图谱及含量测定方法,可为细辛药材不同来源与不同部位的质量评价提供科学依据。

Objective：To establish GC Fingerprint of Herba Asari in different parts and a quantitative analysis method for simultaneous determination of α - pinene, 1 8 - cineole and methyl eugenol of Herba Asari. Methods ： Using capillary gas chromatography on the collected Herba Asari 6 batches in part on the ground, roots and rhizomes for fingerprint analysis ; and use of external standard method simultaneous determination of α - pinene, 1 8 - cineole and methyl eugenol of 34 batches Herba Asari from the eight provinces in China. Results： To establish GC Fingerprint of Herba Asari in different parts of 39 common peaks for indicators. Good linearlty of α -pinene, was showed within the range of 2.58 ~ 46.44ng（ r =0. 9990）, 1 8 -cineole within the range of 2. 292 -32. 088ng（ r = 0.9990） and methyl eugenol within the range of 3. 7768 ~ 151. 872ng（ r = 0.9997 ）. The average recovery were 95.35% （ RSD = 2.38% ）, 96. 11% （ RSD = 5.08% ）, 94. 89% （. RSD = 2.71% ） respectively. Conclusion： The methods of GC Fingerprint and assay of Herba Asari in different parts can provide the scientific basis for quality assessment.