胎盘提取液对脂肪肝大鼠血清TNF-α IL-6水平的影响

Experimental Study on Influence of Human Placenta on TNF-α and IL-6 in Blood Serum of Rats with Nonalcoholic Fatty Liver Disease

目的:探讨人类胎盘提取液对高脂饮食性脂肪肝大鼠血清中TNF-α、IL-6的影响。方法:34只W istar大鼠,雄性,分为正常组、模型组、胎盘提取液高剂量组和低剂量组,予既定试剂4周后,取肝组织行HE染色观察组织学改变,用放免法检测各组大鼠血清中TNF-α、IL-6的含量。结果:模型组血清中TNF-α、IL-6的含量较正常组显著升高(P<0.05或P<0.01),胎盘提取液高、低剂量组较模型组显著降低(P<0.05或P<0.01)。组织学观察模型组均出现了中度以上大泡性肝脂肪变性,胎盘提取液低剂量组较模型组有一定程度的改善,胎盘提取液高剂量组脂肪浸润状态较模型组有明显好转。结论:TNF-α、IL-6参与脂肪性肝病的发生。人类胎盘提取液对高脂饮食性脂肪肝具有显著疗效,作用机制可能与其显著下调血清中TNF-α、IL-6水平有关。

Objective ： To investigate the influence of human placenta in blood serum of rats with nonalcoholic fatty liver disease on TNF - α and IL - 6. Methods ： Thirty - four male Wistar rats were randomly divided into four groups ： normal group, model group, human placenta high dose group and human placenta low dose group. After treated with different agentia already definited for four weeks,histological examinations of liver were carried out with HE staining;meanwhile, the serum levels of TNF-α and IL - 6 were detected with RIA. Results ： Compared with the normal group, the serum levels of TNF- αand IL -6 in the model group was remarkably increased（P 〈0.05 or P 〈 0.01 ）. And the human placenta could significantly lower the levels of TNF -α and IL - 6 （ P 〈 0.05 or P 〈 0.01 ）. Histological examinations showed that there were severe fatty degeneration of hepatocytes in the model group. The damage in livers with low dose human placenta extracts was slighdy milder than that of the models. While high dose human placenta could obviously lessen the fatty degeneration. Conclusion ： TNF-α and IL - 6 were concerned with the pathogenesis of nonalcoholic fatty liver disease. Human placenta is effective in treating fatty liver induced by high - fatty diet. This may be due to its effects in lowering the levels of TNF-α and IL - 6.