盆腔炎颗粒对慢性盆腔炎大鼠血清炎症细胞因子及趋化因子的影响

The Effect of Penqiangyan Granule on Inflammatory Cytokines and Chemokines in Rats with Chronic Pelvic Inflammation

目的:研究盆腔炎颗粒对混合菌加机械刺激所致大鼠慢性盆腔炎的抗炎抗粘连作用机理。方法:以混合细菌加机械刺激法复制大鼠慢性盆腔炎模型,随机分为正常组、模型组、假手术组、盆炎净组及盆腔炎颗粒大、中、小剂量组,造模2周后,各治疗组灌胃给药2周,以酶联免疫吸附法检测各组大鼠血清IL-1β、IL-10、IL-8、MCP-1的浓度。结果:盆腔炎颗粒可以显著降低模型大鼠血清IL-1β、IL-8、MCP-1水平,并显著升高血清IL-10水平,且大、中剂量组接近正常组水平。结论:盆腔炎颗粒对模型大鼠的抗炎抗粘连作用的机理可能是降低血清前炎症因子及趋化因子水平,同时升高抗炎症因子水平,从而恢复炎症因子平衡,减轻局部炎症反应和纤维增生。

Objective ：To explore the anti -inflammation and anti -adhesion effect of Penqiangyan granule on the rats with chronic pelvic inflammation. Methods ： The model was established with injection of mixed bacteria plus mechanic injury. Two weeks later the rats were randomly divided into 7 groups ： normal group, mode/group, sham operation group, Penyanjing group,Penqingyan low dose group, medium close group and high dose group. The intragastric administration was given for 2 weeks . The serous levels of IL - 1β, IL - 8, MCP - 1 and IL - 10 were measured using enzyme - linked immunosorbent assay. Results：Penqiangyan granule could attenuate the rats＇ endometritis, decrease the serous levels of IL - 1β, IL - 8, MCP - 1 and increase the level of IL - 10 significantly. Conclusion ： The anti - inflammation and anti - adhesion effect of Penqiangyan granule may be that it can decrease pro - inflammatory cytokine and chemokine levels and increase anti - inflammatory cytokine levels, thus restore the balance and attenuate inflammation and fibrosis.