血黏度增高致脑细胞膜电位及线粒体膜电位变化

Effects of high blood viscosity on cellular membrane potential and mitochondria membrane potential in brain cells of rats

目的探讨血黏度增高致大鼠脑组织慢性缺血、缺氧后脑细胞膜电位和线粒体膜电位的变化。方法采用高分子右旋糖酐法建立血黏度增高大鼠模型,用血液黏度仪检测全血黏度;以DiBAC_4(3)和罗丹明123为细胞膜电位和线粒体膜电位的荧光指示剂,激光共聚焦显微镜观察并测量血黏度增高大鼠脑细胞悬液中细胞膜和线粒体膜电位荧光强度的变化。结果随注射次数的增加,造模时间延长,全血黏度逐渐升高,与造模时间呈正相关;血黏度增高大鼠脑细胞膜电位和线粒体膜电位变化显著,与造模时间呈时间依赖性关系。结论血黏度增高可导致脑细胞损伤;细胞膜电位和线粒体膜电位变化是造成脑细胞损伤的可能机制。

Objective To investigate the changes of membrane potential and mitochondria mem- brane potential on brain cells of rats after chronic ischemia and hypoxia induced by high blood viscosity, and the mechanism of brain cell damage. Methods Animal model of high blood viscos- ity was established by intravenous injection of high molecular dextran to rats. The whole blood viscosity was detected using automatic programming coagulation analyzer. The alterations of membrane potential and mitochondria membrane potential in brain cells were measured by con- focal laser scanning microscope using DiBAC4 （3） as membrane potential indicator and rhodamine 123 as mitoehondria membrane potential indicator. Results Membrane potential and mitochon- dria membrane potential in brain ceils of rats with high blood viscosity changed markedly, and they were correlated with the blood viscosity. Conclusion Chronic ischemia by high blood vis- cosity can lead to the injury of brain cells, and membrane potential and mitoehondria membrane potential changes are the possible mechanisms of brain cell damage.