用非放射性原位杂交技术定位家猪PGD和HSL基因

ASSIGNMENET OF THE PGD AND HSL GENES IN PIGS BY USING NONRADIOACTIVE in situ HYBRIDIZATION

用地高辛为标记物，以随机引物法标记猪ＰＧＤｃＤＮＡ和ＨＳＬｃＤＮＡ探针，经与半微量全血培养法制备的染色体进行原位杂交后，将猪ＰＧＤ和ＨＳＬ基因分别定位在猪６号染色体６ｑ２５和６ｃｅｎ－６ｑ２１区域。

Porcine partial PGD cDNA and HSL cDNA were labeled with degoxigenin with random primed labeling method, then used in chromosomal in situ hybridization. The porcine PGD and HSL gene were mapped to 6q 25 and 6cen-q 21 , respectively.