摘要
研究了含不同浓度纳米氧化锌的海丝纤维的体外抗金黄色葡萄球菌、大肠埃希菌和白假丝酵母菌的作用,以及纳米氧化锌海丝纤维洗脱液对非洲绿猴肾细胞(Vero细胞)的毒性作用。采用细菌生长抑制的定性和定量试验测定抑菌圈直径及抑菌率;采用MTT法细胞毒性试验测定纳米氧化锌海丝纤维洗脱液体外对Vero细胞的相对增殖率,观察细胞形态学改变,并确定相应毒性等级。结果表明,含0.2%、0.5%、0.8%、1.5%纳米氧化锌的海丝纤维对金黄色葡萄球菌和大肠埃希菌的抑菌圈直径分别为2.35、2.45、2.50、2.75 cm和2.45、2.85、3.05、3.35 cm,抑菌率分别为63.97%、73.02%、88.31%、96.43%和72.50%、83.75%、88.75%、97.58%;对白假丝酵母菌的抑菌圈直径则为2.85、3.00、3.20、3.25 cm。含0.2%、0.5%、0.8%、1.5%纳米氧化锌的海丝纤维洗脱液的毒性反应级别均在2级以下,基本属无细胞毒性,细胞相容性好。
Coli and C. albicans by seacell fibre contained different doses nano - sized ZnO, and the cytotoxicity of Vero ceils by nano- sized ZnO from seacell fibre in vitro were studied. Methods With the qualitative and quantitative of bacteria inhibition growth experiments, the diameters and antibacterial rates of Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, and C. albicans ATCC14053 were determined under the affection of contained different concentrations of ZnO nanoparticles seacell fibre. The cytotoxicity tests of the ZnO nanoparticles on the Vero cell lines were conducted to evaluate the salty with MTr cytotoxicity assay. The result shows that the antibacterial circles diameters of the S. aureus ATCC25923 and E. coli ATCC25922 were 2.35cm, 2.45cm, 2.50cm, 2.75cm and 2.45cm, 2.85cm, 3.05cm, 3.35cm, respectively. The C. albicans antibacterial circlesitiameters were 2.85cm, 3.00cm, 3.20cm, 3.25cm. In the same concentrations of ZnO nanoparticles in the seacell fibre, the rates of inhibition were 63.97%, 73.02%, 88.31%, 96.43% and 72.50%, 83.75%, 88.75%, 97.58%, respectively. Particularly, the antibacterial effect of E. coli ATCC25922 was more significant than S. aureus ATCC25923 in the seacell fibre with 0.2% and 0.5% ZnO nanoparticle ( P 〈 0.05 ). On the other hand, the cytotoxicities of nano - scale ZnO from seacell fibre were all under 2 degree. Conclusion is that the scacell fibre contained nano - sized ZnO has excellent antibacterial effect (especially to E. cob and C. albicans) and less cytotoxicity.
出处
《上海纺织科技》
北大核心
2009年第12期5-7,共3页
Shanghai Textile Science & Technology
基金
浙江省"新苗人才计划"项目(2008R40G2190015)
关键词
纳米氧化锌
海丝纤维
抗菌作用
细胞毒性
测试
nano - sized ZnO
seacell fibre
antibacterial effect
eytotoxicity
testing