NF-κB p65、Caspase-3在重症急性胰腺炎大鼠肝损伤中的表达意义

Role of NF-κB p65,Caspase-3 in the course of liver injury in rat with SAP

目的探讨重症急性胰腺炎大鼠肝损伤中NF-κB p65、Caspase-3表达意义。方法将SD大鼠36只随机分成SAP组(n=18)、假手术组(n=18)。采用改良的Aho’s法制作SAP模型,并经病理及血清学检查证实成功的SAP模型,通过免疫组织化学方法动态观察2组大鼠肝组织中NF-κB p65、Caspase-3的表达,同时观察肝脏病理变化。结果病理观察显示,SAP组大鼠肝组织可见肝细胞明显肿胀、肝窦充血,肝细胞界限不清,肝索扭曲,至12h可见局灶性坏死,并有大量中性粒细胞及淋巴细胞浸润,以小叶区明显。免疫组化检测结果显示在SAP组肝组织中3h、6h、12hNF-κB p65表达分别为(2.83±1.84)、(5.50±1.98)、(8.00±1.55),Caspase-3表达分别为(2.50±0.84)、(5.34±1.16)、(6.17±1.61),其12h与3h比较均有统计学意义(P<0.05);假手术组呈弱阳性表达,并随时间延长阳性表达比较无统计学意义(P>0.05)。不同组别肝脏组织镜下评分、肝组织NF-κB P65及Caspase-3表达经Pearson相关性分析三者成正相关性(P<0.05)。结论NF-κB、Caspase-3升高为SAP肝损伤早期表现,其过度表达在SAP肝脏损伤发病机制中可能发挥重要作用。

Objective To study the role of NF-κB p65,Caspase-3 in the course of liver injury in rat with severe acute pancre- atitis（SAP）.Methods A total of 36 Spragne Dawley rats were randomly and averagely divided into 2 groups, named group A , group C.A and C severed as SAP model and sham operation group, respectively. The modified Aho＇s method was used to reproduce SAP models, and the success of SAP model confirmed by pathology and serum examination,the expression of NF-κB p65,Caspase-3 in liver tissues was determined by immunohistochemical staining and pathological changes in liver were observed.Results The histological examination revealed obviously edema, congestive in hepatic sinusoid, nuclear psychosis, liver cells boundaries unclear, and liver Faso distorted after modeling, Focal necrosis can be seen and a large number of neutrophil and lymphocyte infiltrate in the lobular ar eas at 12h after modeling, In group Aand C, the expression of NF-κB p65 were（2.83±1.84vs2.67±0.52）, （5.50±1.98vs2.17±0.75）and （8.00＋1.55vs2.00±1.10）at the 3rd ,6th ,12th hour, respectively, while in the expression of Caspase-3 were （2.50±0.84vs 2.17±0.75）, （5.34±1.16vs3.17±0.65）, （6.17±1.61vs3.33±1.37）in the same time, respectively, there were significant difference between group A and C at the 12th and 3rd hour （P〈0.05）. Conclusion The increased expression of NF-κB p65 and Caspase-3 were early performance on liver injury in rats with SAP,and the over expressions of them play a pivotal role in the pathogenesis of SAP complicated with liver injury.