三种ELISA方法检测苹果褪绿叶斑病毒和苹果茎沟病毒的比较

COMPARISON OF THREE ELISA METHOD FOR DETECTION OF APPLE CHLOR1TIC LEAFSPOT VIRUS AND APPLE STEM GROOVING VIRUS

苹果褪绿叶斑病毒(ACLSV)和苹果茎沟病毒(ASGV)是感染苹果和其它一些果树的重要病毒。作者应用PAS-ELISA法成功地检测了苹果组培苗中的这两种病毒。为了简化操作步骤,试验了DAS-ELISA和改良DAS-ELISA法,并与PAS-ELISA的检测结果比较。试验结果表明,DAS-ELISA能检测出ASGV,却不能检测出ACLSV,与此同时,这两种病毒均可用改良DAS-ELISA检测。DAS-ELISA与改良DAS-ELISA可缩短检测时间、提高效率,但此方法有较高的株系特异性,因而可能产生假阴性反应。

PAS-ELISA method was successfully used to detect apple chlorotic leafspot virus (ACLSV) and apple stem grooving virus (ASGV), which are important virus diseases in fruit trees, in tissue culture apple tree seedlings. DAS-ELISA and modified DAS-ELISA techniques were compared with PAS-ELISA to simplify the operation procedures. The result showed that DAS-ELISA could detect ASGV but ACLSV. Modified DAS-ELISA could detect both viruses. Both DAS-ELISA and modified DAS-ELISA techniques could shorten the detection time of ACLSV and ASGV. The techniques, however, have higher strain specificity. The false negatives can be caused therefore.