组织蛋白酶S和核转录因子κB在糖尿病大鼠动脉硬化中的表达及意义

EXPRESSION OF CATHEPSIN S AND NUCLEAR FACTOR-KAPPA B IN AORTA OF DIABETIC RATS WITH ATHEROSCLEROSIS

目的探讨糖尿病大鼠大血管组织蛋白酶S(Cat S)和核转录因子κB(NF-κB)的变化。方法25只雄性SD大鼠被随机分为对照组、模型组(1周,4周,8周,12周)。采用腹腔注射链脲霉素(STZ)方法复制大鼠糖尿病模型,HE染色观察主动脉管壁形态学变化,分别采用Western-blotting和RT-PCR方法检测大血管Cat S、NF-κB蛋白和mRNA的变化。结果造模后8周可见管壁结构的变化。造模后4周,Cat S蛋白和mRNA开始增加,8周达到高峰。NF-κB蛋白和mRNA在造模后1周开始增加,4周后达到表达高峰。结论Cat S和NF-κB在糖尿病大血管病变过程中起重要作用。

Objective To investigate the expression of cathepsin S （Cat S）and nuclear factor- kappa B （NF- κB）in ath- erosclerosis of aorta in diabetic rats. Methods 25 male SD rats were randomly divided into control group and model groups （1w,4w,8w,12w）. STZ was used to induce diabetic and HE staining was used to observe the structure of the artery. The protein and mRNA expression of Cat S and NF - κB were detected by Western - blotting and RT - PCR. Results The protein and mRNA levels of Cat S in diabetic rats increased after 4 weeks since diabetic model establishing, and the levles of NF - κB increased after 1 week. Conclusion Cat S and NF-κB play important roles in athero- sclerosis of diabetic.