满天星微繁技术研究

A STUDY ON THE TECHNIQUE OF MICROREPRODUCTION OF GYPSOPHILA PANICULATA

用无病毒满天星的带芽茎段作为外植体，研究其组培试管苗生长协调性。结果表明：在起始培养中，以ＭＳ＋ＢＡ２．５ｍｇ／Ｌ＋ＮＡＡ０．０１ｍｇ／Ｌ培养基为最好，侧芽诱导率最高，平均为１８个；在增殖培养中，低世代使用ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ培养基为最佳，增殖倍数为１８～２５倍，在高世代使用ＭＳ＋ＢＡ１．５ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ培养基为最佳，增殖倍数在４０倍左右且兼顾了试管苗的粗细、节间长度和愈伤组织块的大小，使之生长协调性良好；在根的诱导中，使用１／２ＭＳ＋ＮＡＡ０．３ｍｇ／Ｌ培养基最易诱导生根且根系发达。

By using segments of the stems with buds of non virus Gypsophila paniculata as explants, the growth harmonization of the young plants cultured in test tube has been studied. The results suggest that: in the primary culture, MS+BA 2.0 mg/L+NAA 0.2 mg/L mcdium shows the best result, the inductive rate of the lateral buds is the highest, the mean value is 1 8; in the subcuture, MS+BA 2.0 mg/L+NAA 0.2 mg/L medium in the primary generations may increase their rates to about 18～25 times; the best medium in the late generations is MS+BA 1.5 mg/L+NAA 0.05 mg/L which may increase their rates to about 40 times, harmonize their degrees of thicknesses, their intercode lengths and their callus sizes of the young plants, and make their growths harmonious; in the root reduction, 1/2MS+NAA 0.3 mg/L medium may very easily induce their root developments, and help their root systems develop greatly.