小鼠腔前卵泡卵母细胞的采集、体外发育和体外成熟

COLLECTION, IVD AND IVM OF PREANTRAL FOLLICULAR OOCYTES IN MOUSE

采用机械法分离成年小鼠和１３日龄小鼠腔前卵泡，两组中每只小鼠平均回收率分别为４９２９±１０８１和７６８３±９１５个；腔前卵泡卵母细胞培养１０天后，两组恢复成熟分裂卵的比例分别为１９３％和２０９％。试验结果表明，小鼠腔前卵泡卵母细胞在培养后第５天开始发生ＧＶＢＤ，此后，ＧＶＢＤ和抛出ｐｂＩ的卵母细胞数量逐日增加。

Preantral follicles were isolated from mouse ovaries by the mechanical method. In adult and 13-day-old mouse, the average recovery rate of preantral follicles was 49·29±10 81 and 76 83±9 15 per mouse, respectively. After the oocytes from preantral follicles were cultured for 10 days, the percentage of oocytes resuming meiosis in these two groups was 19 3% and 20 9%, respectively. It shows that GVBD began to occur in mouse oocytes from preantral follicles after culture on the 5th day in vitro. Thereafter, the number of oocytes generated GVBD and pbI increased day by day.