血浆同型半胱氨酸、ET和NO水平与妊娠期高血压疾病的相关性研究

Blood Plasma ET,NO and Homocysteine in Patients with Pregnancy-induced Hypetension Syndrome

目的探讨血浆同型半胱氨酸(Hcy)与妊娠期高血压疾病(PIH)的关系及内皮素(ET)和一氧化氮(NO)在PIH中的作用。方法选择PIH患者90例,其中妊娠高血压非子痫组36例、子痫前期(轻度)组30例,子痫前期(重度)组24例;并随机选取同期的正常晚期妊娠40例为对照组。分别用荧光偏振免疫分析法测定血浆同型半胱氨酸水平,采用放射免疫分析法和化学法检测血浆ET和NO水平。结果妊娠期高血压疾病患者血浆Hcy和ET水平显著高于对照组,血浆NO值显著低于对照组(P<0.05),以子痫前期(重度)组增高明显;血浆Hcy水平和ET呈正相关,和NO呈负相关。结论妊娠期高血压疾病患者血浆Hcy异常升高引起了血管内皮损伤,ET的异常增高和NO减少,导致ET与NO功能失调,血压升高,可能是促进了妊娠期高血压疾病形成重要机制之一。

Objective To investigate the relation between the homocysteine and pregnancy-induced hypetension syndrome （PIH） as well as the mechanisms of blood plasma endothelin, nitrogen monoxidum in PIH. Methods 90 PIH women included 36 gestational hypertension, 30 mild preeclampsias , and 24 severe preeclampsias, and another 40 normal late-term pregnant women as controls were recruited in this study. The plasma Hcy were determined by fluorescence polarization immunoassay and plasma endothelin, nitrogen monoxidum were determined by radio immunoassay and chemical method, respectively. Results The plasma Hcy and ET levels in PIH was significantly higher those in controls as well as the plasma NO was significantly lower in PIH than that in control group, especially in the those with preeclampsia. The plasma Hcy level had positive correlation with plasma ET level and had negative correlation with plasma NO level in PIH. Conclusion The Hcy in plasma which resulted in the increase of plasma ET and NO level can be regarded as the important mechanisms of inducing the development of PIH.