摘要
文章报道了整体荧光原位杂交应用于检测水稻特异DNA的实验程序利用该实验方法,以经地高辛(digoxin,DIG)标记的rDNA作为探针,成功地在水稻根尖和花药内部组织检测到与探针互补的片段对于实验方法,结果表明,整体荧光原位杂交的成功与否受到多种因素的影响,包括组织的种类、固定剂的种类以及固定后的处理方法(主要是酶解与否)等检测根尖DNA可以采用φ(福尔马林)=1%固定加上酶解;检测花药(花粉发育处于单核期)DNA也可以采用φ(福尔马林)=1%固定。
A procedure of whole mount flurescence in situ hybridization (WFISH) for detecting specific DNA in rice was reported. The techique was demonstrated on root and anther of rice with rDNA as probe, labelled by DIG. It was found that the hybridization efficiency depended on the methods further used after fixation, types of tissue and of fixative. Fixation in 1% formalin plus enzyme digestion could yield excellent results of DNA:DNA in root tips. Fixation in 1% formalin (no need enzyme digestion) yielded the excellent results with respect to morphology and hybridization efficiency in rice anther.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1998年第3期1-4,共4页
Journal of South China Agricultural University
基金
香港大学UGC Block Grant资助
关键词
水稻
荧光原位杂交
DNA
遗传育种
rice
whole mount flurescence in situ hybridization (WFISH)
rDNA