β-葡萄糖苷酸酶在豚鼠组织中的分布

DISTRIBUTION OF β-GLUCURONIDASE ACTIVITY IN GUINEA PIG TISSUES

利用“后偶合技术”对正常豚鼠主要组织中β－葡萄糖苷酸酶（β－ＧＣＤ）的分布与含量进行了研究结果表明：肾上腺、脾脏、肝脏、睾丸、卵巢、肾脏和淋巴结的大多数实质细胞中富含β－ＧＣＤ而脑、肺、肌肉、胃肠和胰腺的实质细胞染色较弱组织中呈深染的细胞为巨噬细胞、肝和肾的实质细胞及某些粘液细胞该项工作以豚鼠作为动物模型而研究酶代谢紊乱。

The histochemical distribution of β glucuronidase activity in guinea pig tissues was demonstration by postcoupling technique.The present study showed that the enzyme rich tissues were the liver,kidney,thyroid,lymph node and ovary.The enzyme poor tissues were the brain,testis and muscle.Macrophages,parenchymal cells of liver and kidney and several kinds of muciferous cells were intensely stained in histological sections.This study provides information on the normal distribution of β glucuronidase,which will be useful when using the guinea pig as a model in enzyme metabolism disturbance studies.