云杉种子超干贮藏的生理生化特征

Effects of Ultra-Dry Treatment on Physiological and Biochemical Characteristics of Picea asperata Seeds

采用硅胶室温干燥法,云杉种子含水量由82g.kg降至64,52,41,29g.kg。测定不同含水量种子的发芽势、发芽率、发芽指数,并测定种子老化(50℃,100%RH)后的相对电导率、脱氢酶活性和异柠檬酸裂解酶活性等生理生化指标,以研究超干处理对种子活力和种子生理特性的影响。结果表明:云杉种子有较高耐脱水性,含水量降至41g.kg-1,种子活力仍维持在较高的水平,细胞膜能够保持较好的完整性,脱氢酶活性、异柠檬酸裂解酶活性与对照没有显著差异。利用超干技术保存云杉种子是可行的。

Seeds of Picea asperata were dried from 82 g·kg-1 to 64,52,41 and 29 g·kg-1 moisture content in a desiccator with silica gel.After the desiccation,seed vigor and physiological indices,including seed germination energy,germination percentage,germination index,relative conductivity,dehydrogenase activity and isocitrate lyase activity were measured.The results showed that the seeds had high desiccation tolerance.When dried to 41 g·kg-1,the seeds still had stable viability,the relatively integral cell membrane structure,the high dehydrogenase and isocitrate lyase activities.To some extent,the seed kept genetic stability by artificial aging test.