摘要
建立了秦艽中獐牙菜苦苷含量测定的RP-HPLC方法。色谱条件:采用ZorbaxEclipseXDB-C18柱(150mm×4.6mm,5μm),流动相:甲醇-0.03%磷酸水溶液梯度洗脱(0～25min:15%～23%;25～30min:23%～30%),流速:1mL/min,柱温:25℃,检测波长240nm,獐牙菜苦苷在0.20～1.8μg范围内成良好的线性关系,r=0.9999,回收率RSD=1.32%。对青海产4种秦艽中獐牙菜苦苷的含量进行了定量分析,测定结果:小秦艽中獐牙菜苦苷的含量最高为:0.62%,麻花秦艽与管花秦艽的含量没有显著差异,分别为:0.43%,0.45%,簧管秦艽的含量最低为:0.32%。
A method was established for the determination of swertiamarin by RP - HPLC. Experiments were performed on Zorbax Eclipse XDB - C18 （4.6 mm × 250 mm,5 μm） column, with CH3OH - 0. 03%H3PO4（0-25 min： 15% -23% ;25 -30 rain：23%- 30% ）as mobile phase at a flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the determination wavelength was 240 nm. The linear range was 0.20 μg - 1.8 μg（ r = 0.999 9） and the average recovery was 98.6% （RSD = 1.32% ）. The contents of swertiamarin were detected, and the results were relevant to the source. The content of swertiamarin of Gentiana dahurica Fisch. was 0.62%, the content of Gentiana straminea Maxim. and Gentiana siPhonantha Maxim. ex Kusnez are 0.43% ,0.45%, Gentiana officinalis H. Smith is 0.32%.
出处
《中国野生植物资源》
2009年第5期51-53,共3页
Chinese Wild Plant Resources
基金
科技部国家科技支撑计划(2007BAI45B00)
