SD大鼠左心室利钠肽三型受体的表达

Expression of three natriuretic peptide receptor subtypes on the left ventricle in sprague-dawley rats

目的:研究利钠肽三种亚型受体(NPR-A,NPR-B和NPR-C)是否在正常SD大鼠左心室心肌细胞有表达。方法:应用免疫组织化学和Western免疫印迹检测SD大鼠左室心肌NPR-A、NPR-B和NPR-C的表达。结果:两种方法显示SD大鼠左室心肌有NPR-A、NPR-B和NPR-C三种亚型受体的表达,以NPR-A表达最强,NPR-C表达最弱。结论:正常SD大鼠左室心肌有A、B、C三种利钠肽受体表达。

Objective： To study whether all three natriuretic peptide receptor （NPR） subtypes （NPR--A, NPR--B, and NPR--C） are expressed on the myocytes of left ventricle （LV） of normal Sprague--Dawley （SD） rats. Methods： Both immunohistochemistry staining and Western blotting were applied to detect the expression of NPR--A, NPR-- B, and NPR--C on myocytes. Results： The receptors of NPR--A, NPR--B, and NPR--C were expressed on the myocytes of LV. Furthermore, the expression of NPR-- A was the highest but the NPR-- C was the lowest on the myocytes. Conclusion： All Three subtypes of NPR are expressed on the myocytes of LV in normal SD rats.