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自噬相关基因Beclin1在星形细胞瘤中的表达及意义 被引量：5

Expression and clinical significance of autophagy-related gene Beclin 1 in astrocytic tumors
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摘要 目的探讨自噬相关基因Beclin1在星形细胞瘤中的表达,及与肿瘤病理和临床表现的相关性与意义。方法采用免疫组化和免疫印迹法检测62例不同级别的星形细胞瘤Beclin1的表达情况,并结合临床表现和病理结果进行相关性分析。结果免疫组化显示星形细胞瘤中Beclin1蛋白表达随肿瘤级别升高而降低。免疫印迹法显示高级别(Ⅲ～Ⅳ级)星形细胞瘤Beclin1平均光密度比值低于低级别(Ⅰ～Ⅱ级)肿瘤(P=0.036)。生存期大于36个月者Beclin1平均光密度比值高于生存期小于36个月者(P=0.041)。结论Beclin1在高级别星形细胞瘤中表达下降,Beclin1检测可作为判断生存期和临床预后的辅助指标。 Objective To explore the expression of autophagy-related gene Beclin 1 and its correlations with tumor pathophysiology, clinical manifestations in astrocytic tumors. Methods Expression ofBeclin 1 in 62 astrocytic tumor specimens with different pathological grades was detected by irnmunohistochemistry and Western-blotting. The correlations ofBeclin 1 expression to clinicopathology and clinical characteristics of the patients were analyzed. Results Immunohistochemistry showed a decrease ofBeclin 1 protein expression in differentgrade astrocytie tumors. Western-blotting indicated that the average optical density ratio in high-grade astrocytic tumor （grade Ⅲ -Ⅳ） was lower than that in low-grade astrocytic tumor （grade Ⅰ - Ⅱ, P= 0.036）. The average optical density ratio in long survival time （≥36 months） patients was higher than that in short survival time （〈 36 months） patients （P= 0.041）. Conclusion Beclin 1 is down-regulated in high-grade astrocytic tumors and can be used as an auxiliary index in determination of survival period and prediction of clinical outcome in patients with astrocytic tumors.
作者 黄新 白红民 陈亮 李宾 卢亦成
机构地区 中国人民解放军第二军医大学附属长征医院神经外科 中国人民解放军广州军区广州总医院神经外科 南方医科大学神经生物教研室
出处 《中国微侵袭神经外科杂志》 CAS 北大核心 2009年第12期559-562,共4页 Chinese Journal of Minimally Invasive Neurosurgery
关键词 星形细胞瘤 自噬 Beclin1基因 生存期 astrocytoma autophagy Beclin 1 gene survival
分类号 R739.41 [医药卫生—肿瘤] R730.264 [医药卫生—肿瘤]
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参考文献15

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共引文献53

同被引文献38

引证文献5

二级引证文献18

中国微侵袭神经外科杂志
2009年 第12期

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