湖北土家族群体15个短串联重复序列基因座遗传多态性

Genetic polymorphisms of fifteen short tandem repeats in Tujia ethnic group of Habei province

目的调查湖北地区土家族人群15个短串联重复序列（shorttandemrepeat，STR）基因座遗传多态性分布和群体遗传学数据，比较分析湖北土家族与重庆土家族等位基因的分布频率。方法采用毛细管电泳技术和五色荧光复合扩增的方法，对湖北土家族333名无关个体的15个STR基因座进行基因分型。结果共检出151个等位基因，多于重庆土家族的等位基因检出数（141个），其频率分布在0．002～0．498之间。经Statisticalgenetics软件分析，各基因座的群体基因型分布符合Hardy—Weinberg平衡（P〉0．05）。15个STR基因座的杂合度在0．652-0．867之间，个体识别力在0．802～0．971之间，累积个体识别力大于0．9999999；非父排除率在0．357～0．730之间，累积非父排除率为0．9999997；多态信息含量在0．57～0．87之间。结论建立了湖北土家族人群的STR基因座的群体资料，为土家族人群的群体遗传学研究、法医个人识别、亲子鉴定提供了基础数据；湖北土家族与重庆土家族亲缘关系相近，等位基因频率差异无统计学意义（P〉0．05），但在等位基因检出数及部分基因分布频率上仍存在差异。

Objective To investigate the genetic polymorphisms of 15 short tandem repeat （STR） loci in Tujia ethnic group of Hubei province, and to compare the allele frequencies between Hubei Tujia and Chongqing Tujia populations. Methods Capillary electrophoresis and five-color fluorescent multiamplification were applied to detect the genotypes of 15 STR loci in 333 unrelated Hubei Tujia individuals. Results One hundred and fifty one alleles were observed in the population, which is higher than 141 alleles observed in Chongqin Tujia population, and their frequencies were from 0. 002 to 0. 498. No significant deviation from the Hard-Weinberg Equilibrium was observed （P;〉0.05）. Statistical analysis showed that the heterozygosity of the 15 loci ranged from 0. 652 to 0. 867. The values of discrimination power （DP） ranged from 0. 802 to 0. 971. The values of excluding probability of paternity （EPP） ranged from 0. 357 to 0. 730, and the polymorphism information content （PIC） was 0. 57 0. 87. The fifteen loci showed an accumulated total discrimination power （TDP） of more than 0. 999 999 9, and the cumulative excluding probability of paternity was 0. 999 999 7. Conclusion This study has accumulated the population genetic data of the 15 STRs in Hubei Tujia ethnic group. The data can be used in forensic and individual identification for this population. Hubei Tujia is phylogenetically related to Chongqing Tujia, and the allele frequencies were non-distinctive （P〉 0. 05）, while the number of alleles detected, and the frequencies of some alleles observed were different.