磁性壳聚糖复合微球的制备及表征

Preparation and Characterization of Magnetic Chitosan Compound Microspheres

采用磁性Fe_3O_4纳米颗粒为磁核,用反相悬浮聚合法制备了磁性壳聚糖复合微球,用激光粒度仪和透射电镜对磁性壳聚糖微球的尺寸和形貌进行分析,考察了壳聚糖浓度、乳化剂用量、交联剂浓度、搅拌速度等参数对壳聚糖成球的影响。结果表明:使用4 mL质量浓度为4 g·L^(-1)壳聚糖溶液、0.1 mL司本-80和0.1 mg磁性Fe_3O_4纳米颗粒,经超声分散1 h后,加入0.5 mL4%的戊二醛,在转速350 r·min^(-1)搅拌条件下,室温下反应2 h,可得到形貌光滑、粒径为0.5～2 μm的磁性壳聚糖复合微球,该壳聚糖复合微球具有较好的磁响应性能。

With Fe3O4 magnetic nanoparticles as magnetic nuclear, the magnetic chitosan microspheres were prepared by means of inverse suspension polymerization. The size and shape of magnetic chitosan microspheres were characterized by laser particle size analysis and TEM, respectively. The effects of the chitosan concentration, the emulsifier amount, the crosslinking reagent concentration and stirring speed on chitosan microspheres were investigated. The results show that the magnetie chitosan microspheres with smooth surface morphology and particle size in the 0. 5 -- 2 μm could be prepared by using 4 mL chitosan solution of 4 g · L-1 , added 0. 1 mL Span-80 and 0. 1 mg of the Fe304 magnetic microsphere, which was ultrsonic dispersed for 1 h, and then added into 0. 5 mL of 4% glutaraldehyde under the stirring condition of 350 r · min-1 to react for 2 h at room temperature. The chitosan composite microspheres had better magnetic responsiveness.