摘要
为了建立一种简便、快速、可靠和经济的牛γ干扰素(BoIFN-γ)的免疫学检测方法,本研究通过杂交瘤技术建立了2株抗rBoIFN-γ单克隆抗体(MAb)2G6和5F9。Western blot、抗病毒活性阻断实验表明2株MAb与rBoIFN-γ有高亲和活性。相加ELISA表明2G6和5F9识别rBoIFN-γ不同的抗原表位。利用2G6作为捕获抗体,5F9-HRP作为检测抗体建立的抗原捕获ELISA方法可特异性的检测不同系统表达的rBoIFN-γ,而且与rBoIFN-α、rBoIFN-β不发生交叉反应。该方法的建立为抗原捕获ELISA定量检测BoIFN-γ试剂盒的研制奠定了基础。
An antigen capture ELISA was established by utilizing high affinity monoclonal antibodies （MAb） against BoIFN-γ. Two hybridomas 2G6 and 5F9 were developed and confirmed by western blot and antiviral blocking assay. The additive ELISA demonstrated that the two MAbs recognized different antigenic regions. Antigen capture ELISA based on these MAbs is highly specific and sensitive, proving a quick and reliable method for detecting BoIFN-γ.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第12期953-957,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"攻关"项目(2004BA519A19
2005BA711A10)
国家"973"项目(2005CB523200)
