骨髓移植对小鼠芥子气中毒的治疗作用

Therapeutic effect of bone marrow transplantation on mustard-poisoned mice

目的将骨髓移植应用于芥子气全身吸收中毒小鼠的治疗,探索芥子气中毒治疗的新方法。方法Balb/c小鼠sc芥子气20mg.kg-1染毒,建立芥子气诱导的造血系统抑制模型。染毒后4h、第1天和第2天3次尾静脉输注雄性小鼠骨髓细胞悬液(每次约1×106细胞),染毒后第3,5,7和10天取血和股骨,计数外周血白细胞和骨髓有核细胞。另一组实验小鼠在染毒后4h及次日2次尾静脉输注骨髓细胞的同时合并使用重组粒细胞集落刺激因子(G-CSF)74μg.kg-1,观察上述指标的变化。结果与模型组比较,骨髓移植组小鼠血白细胞数呈升高趋势,至第10天有显著性差异;骨髓有核细胞数量升高明显,从第5天起有显著性差异。伍用G-CSF后,早期即可显著升高白细胞数。结论骨髓移植可有效改善芥子气中毒引起的造血系统抑制。

AIM To study the therapeutic effect of bone marrow transplantation on mustard gas-poisoned mice.METHODS Mustard-poisoned mice model was constructed by injected mustard gas 20 mg·kg-1 subcutaneously.At 4 h,1 d and 2 d after poisoned by mustard gas,the suspended bone marrow cells（106 cells per time） were injected into mice respectively.After poisoned 3,5,7 and 10 d,the numbers of leukocyte and marrow nucleated cells were recorded periodically.At the same time,after poisoned 4 h and 1 d by mustard gas,mice in another group were injected granulocyte colony stimulating factor（G-CSF） 74 μg·kg-1combined with transplantation and changes of the cell number were observed.RESULTS Compared with poisoned group without transplantion,leukocyte number in bone marrow transplanted group presented ascending trend,but there was no statistical significance until the 10 d after poisoned.However,the number of marrow nucleated cell increased significantly from the 5th day.After mice injected bone marrow cells combined with G-CSF,leukocyte increased markedly in earlier stage compared with transplantation only.CONCLUTION The bone marrow transplantation has significant effects on improvement of marrow hematogenic system inhibited by mustard gas.